In vitro antimicrobial and antioxidant activity of biogenically synthesized palladium and platinum nanoparticles using botryococcus braunii

INTRODUCTION: Context: The spreading of infectious diseases and the increase in the drug resistant among microbes has forced the researchers to synthesize biologically active nanoparticles. Improvement of eco-friendly procedure for the synthesis of nanoparticles is growing day by day in the field of nanobiotechnology.
Objectives: In the present study we use the extract of green alga Botryococcus braunii for the synthesis of palladium and platinum nanoparticles and evaluation of their antimicrobial and antioxidant activity.
METHODS: Methods and Material: Green alga was collected from Udaisagar Lake Udaipur (Rajasthan, India) and isolated by serial dilution method and grown on Chu-13 nutrient medium. The characterization of alga synthesized palladium and platinum nanoparticles was carried out by using X-ray diffraction and scanning electron spectroscopy. The zone of inhibition was measured by agar well plate method and minimum inhibitory concentration was determined by agar dilution assay for antimicrobial activity. Antioxidant activity of nanoparticles was also studied by DPPH method.
RESULTS: Results: Stable palladium and platinum nanoparticles were successfully produced by using green alga. XRD pattern revealed about crystalline nature and SEM micrographs have shown the morphology of biogenically synthesized metal nanoparticles.FT-IR measurements showed all functional groups having control over stabilization and reduction of the nanoparticles. Green synthesized nanoparticles exhibited antimicrobial activity against gram positive and gram negatives bacterial strains, antifungal activity against fungus and also demonstrates antioxidant activity.
DISCUSSION AND CONCLUSION: Conclusion: The biogenic synthesis of metal nanoparticles can be promising process for production of other transition metal nanoparticles and also new nanocatalyst will revolutionise the synthesis of organic heterocycles.