Antioxidant Properties Of Rubus Discolor L. Extracts And Protective Effects Of Its Flower Extract Against Hydrogen Peroxide-Induced Oxidative Stress In Wistar Rats
Serhat Keser1, Sait Çelik2, Semra Turkoglu3, Ökkes Yılmaz4, Ismail Turkoglu51Firat University, Faculty Of Science, Chemistry Department 23119-elazig/turkey2Usak University, Faculty Of Dentistry 64000-usak/turkey
3Tunceli University, Faculty Of Engineering, Food Engineering Department 62000-tunceli/turkey
4Firat University, Faculty Of Science, Biology Department 23119-elazig/turkey
5Firat University, Education Faculty, Department Of Biology Education 23119-elazig/turkey
In the present study, in vitro antioxidant activities of ethanol and water extracts of Rubus discolor L. leaves, flower, unripe and ripe fruits were investigated. Additionally, the antioxidant and protective properties of R. discolor flower (RD) water extract on some important biochemical parameters against hydrogen peroxide (HP)-induced oxidative stress in rats were determined. The phytochemical profiles and antioxidant properties (scavenging against DPPH•, ABTS•+, H2O2, O2-, metal chelating, inhibition of lipid peroxidation and reducing power) of extracts were determined by spectrophotometric methods and HPLC. The antioxidant potential of RD flower extract supplementations were evaluated by measuring total protein, glutathione (GSH), malondialdehyde (MDA), vitamin A and E, cholesterol, glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) levels in various tissues. The flower extracts were the most active extracts for in vitro antioxidant assays. In HP administered groups, MDA level was increased, GSH and total protein levels were decreased. In RD treated group, these substances levels were protected. In RD plus HP treated group, SOD and GSH-Px levels were increased. These results show that RD flower extract have the protective effects against H2O2 via its free radical scavenging activity, and this extract affected the level of antioxidant enzymes, vitamin A and E, cholesterol, GSH, total protein and MDA.
Keywords: Rubus discolor, MDA, GSH, SOD, hydrogen peroxide, cholesterolRubus Discolor L. Ekstrelerinin Antioksidan Özellikleri Ve Çiçek Ekstresinin Hidrojen Peroksit-Nedenli Oksidatif Stres Oluşturulmuş Wıstar Sıçanlarda Koruyucu Etkileri
Serhat Keser1, Sait Çelik2, Semra Turkoglu3, Ökkes Yılmaz4, Ismail Turkoglu51Firat University, Faculty Of Science, Chemistry Department 23119-elazig/turkey2Usak University, Faculty Of Dentistry 64000-usak/turkey
3Tunceli University, Faculty Of Engineering, Food Engineering Department 62000-tunceli/turkey
4Firat University, Faculty Of Science, Biology Department 23119-elazig/turkey
5Firat University, Education Faculty, Department Of Biology Education 23119-elazig/turkey
Sunulan çalışmada, Rubus discolor L. yaprak, çiçek, ham meyve ve olgun meyvelerinin etanol ve sulu ekstrelerinin in vitro antioksidan aktiviteleri incelenmiş ve R. discolor çiçek su (RD) ekstresinin hidrojen peroksit (HP)-nedenli oksidatif stres oluşturulmuş sıçanlarda bazı önemli biyokimyasal parametreler üzerindeki antioksidan ve koruyucu etkileri saptanmıştır. Ekstrelerin fitokimyasal profili ve antioksidan özellikleri (DPPH•, ABTS•+, H2O2, O2- yok edici, metal şelatlama, lipit peroksidasyonun inhibisyonu ve indirgeme kuvveti aktiviteleri) spektrofotometrik metotlar ve HPLC cihazıyla belirlendi. RD çiçek su ekstresi uygulamasının antioksidan potansiyeli çeşitli dokularda total protein, glutatyon (GSH), malondialdehit (MDA), A ve E vitaminleri, kolesterol, glutatyon peroksidaz (GSH-Px), süperoksit dismutaz (SOD) seviyelerinin ölçülmesiyle değerlendirildi. In vitro antioksidan testlerde çiçek ekstrelerinin en aktif ekstreler olduğu belirlendi. HP uygulanan gruplarda, MDA seviyesi artmışken, GSH ve total protein seviyeleri azalmıştır. RD uygulanan grupta ise bu maddelerin seviyeleri korunmuştur. RD + HP uygulanan grupta SOD ve GSH-Px seviyeleri artmıştır. Bu sonuçlar göstermiştir ki RD çiçek ekstresi H2O2’e karşı serbest radikal yok etme aktivitesi sayesinde koruyucu etkilere sahiptir ve bu ekstre antioksidan enzimlerle, vitamin A ve E, kolesterol, GSH, total protein ve MDA seviyelerini etkilemiştir.
Anahtar Kelimeler: Rubus discolor, MDA, GSH, SOD, hidrojen peroksit, kolesterolCorresponding Author: Serhat Keser, Türkiye
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