. 2015; 12(2): 146-155 | DOI: 10.5505/tjps.2015.48658 | |||
Evaluation of Antioxidant and Antimicrobial Activities of Tamus communis L. ssp. cretica (L.) Kit Tan and Its Mineral CompositionNurgün Küçükboyacı1, Nilüfer N Turan Dural2, Ayşegül Köroğlu3, Gökalp İşcan4, Ahmet Aydın51Gazi University, Faculty Of Pharmacy, Department Of Pharmacognosy, 06330 Ankara, Turkey,2Gazi University, Faculty Of Pharmacy, Department Of Pharmacology, 06330 Ankara, Turkey 3Ankara University, Faculty Of Pharmacy, Department Of Pharmaceutical Botany, 06100 Ankara, Turkey 4Anadolu University, Faculty Of Pharmacy, Department Of Pharmacognosy, 26470 Eskişehir, Turkey 5Yeditepe University, Faculty Of Pharmacy, Kayışdağı, Ataşehir, 34755 İstanbul, Turkey The aim of the present work was to examine antioxidant and antimicrobial properties of Tamus communis L. ssp. cretica (L.) Kit Tan (Dioscoreaceae). The applied methods for the antioxidant activity of aqueous extract from the aerial parts of the plant were 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging, flow injection analysis-luminol chemiluminescence (FIA-CL) and thiobarbituric acid (TBA) assays. Total phenolic content of the aqueous extract was determined with spectrophotometric method. The antimicrobial activity of the n-hexane, chloroform, ethyl acetate, and ethanol extracts of the aerial parts of the plant was assessed towards selected bacteria. In addition, mineral composition of the raw plant was examined by atomic absorption spectroscopy. Aqueous extract of T. communis ssp. cretica was found to possess DPPH free radical scavenging activity (IC50= 2.85±1.30 mg/mL), inhibitory effect on H2O2- and HOCl-luminol chemiluminescence (-log IC50=3.8±0.09 and IC50=1.3x10-3±4.9x10-4 mg/mL), and inhibitory activity toward lipid peroxidation (IC50=3.82±1.67 μg/mL) compared to the references. Total phenolic content of the aqueous extract was found to be 56.66±0.21 mg gallic acid/g extract. The n-hexane, chloroform, ethyl acetate, and ethanol extracts showed moderate activity having MIC values of 250-500 μg/mL against selected microorganisms. According to our results of mineral composition, the raw plant has low amount of toxic elements (Pb, Cd and Al), macro (Ca and Mg), and microelements (Cu, Fe, Mn and Zn). Keywords: Tamus communis ssp. cretica, Dioscoreaceae, Antioxidant activity, Antimicrobial activity, Mineral analysisTamus communis L. ssp. cretica (L.) Kit Tan’ın Antioksidan ve Antimikrobiyal Aktivitelerinin Değerlendirilmesi ve Mineral KompozisyonuNurgün Küçükboyacı1, Nilüfer N Turan Dural2, Ayşegül Köroğlu3, Gökalp İşcan4, Ahmet Aydın51Gazi University, Faculty Of Pharmacy, Department Of Pharmacognosy, 06330 Ankara, Turkey,2Gazi University, Faculty Of Pharmacy, Department Of Pharmacology, 06330 Ankara, Turkey 3Ankara University, Faculty Of Pharmacy, Department Of Pharmaceutical Botany, 06100 Ankara, Turkey 4Anadolu University, Faculty Of Pharmacy, Department Of Pharmacognosy, 26470 Eskişehir, Turkey 5Yeditepe University, Faculty Of Pharmacy, Kayışdağı, Ataşehir, 34755 İstanbul, Turkey Bu çalışmanın amacı Tamus communis L. ssp. cretica (L.) Kit Tan’ın (Dioscoreaceae) antioksidan ve antimikrobiyal özelliklerinin incelenmesidir. Bitkinin toprak üstü kısımlarının sulu ekstresinin antioksidan aktivitesi için uygulanan metotlar 1,1-difenil-2-pikrilhidrazil (DPPH) süpürücü, akışa injeksiyon analiz-luminol kemiluminesens (FIA-CL) ve tiyobarbitürik asit (TBA) test sistemleridir. Sulu ekstrenin total fenolik içeriği spektrofotometrik metotla tayin edilmiştir. Bitkinin toprak üstü kısımlarının n-hekzan, kloroform, etil asetat ve etanol ekstrelerinin antimikrobiyal aktivitesi seçilen bakterilere karşı incelenmiştir. Ayrıca taze bitkinin mineral bileşimi atomik absorbsiyon spektroskopisi ile belirlenmiştir. T. communis ssp. cretica'nın sulu ekstresi referanslarla karşılaştırıldığında DPPH serbest radikal süpürücü aktiviteye (IC50= 2.85±1.30 mg/mL), H2O2- ve HOCl-luminol kemiluminesens üzerine inhibitör etkiye (-log IC50=3.8±0.09 ve IC50=1.3x10-3±4.9x10-4 mg/mL) ve lipid peroksidasyona karşı inhibitör etkiye (IC50=3.82±1.67 μg/mL) sahip bulunmuştur. Sulu ekstrenin total fenolik içeriği 56.66±0.21 mg gallik asit/g ekstre olarak belirlenmiştir. n-Hekzan, kloroform, etil asetat ve etanol ekstreleri seçilen mikroorganizmalara karşı 250-500 μg/mL MIC değeri ile orta derecede aktiviteye sahip bulunmuştur. Mineral bileşimi sonuçlarına göre, taze bitki düşük miktarda toksik elementler (Pb, Cg ve Al), makro (Ca ve Mg) ve mikroelementleri (Cu, Fe, Mn ve Zn) içermektedir. Anahtar Kelimeler: Tamus communis ssp. cretica, Dioscoreaceae, Antioksidan aktivite, Antimikrobiyal aktivite, Mineral analiziNurgün Küçükboyacı, Nilüfer N Turan Dural, Ayşegül Köroğlu, Gökalp İşcan, Ahmet Aydın. Evaluation of Antioxidant and Antimicrobial Activities of Tamus communis L. ssp. cretica (L.) Kit Tan and Its Mineral Composition. . 2015; 12(2): 146-155 Corresponding Author: Nurgün Küçükboyacı, Türkiye |
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