Quantification of Shikimic Acid in the Methanolic Extracts of Three Alnus Taxons Growing in Turkey
Çiğdem Altınyay1, Burçin Ergene Öz2, Mehmet Levent Altun21The Ministry Of Health Of Turkey, Turkish Medicines And Medical Devices Agency, Ankara, Turkey2Ankara University, Faculty Of Pharmacy, Department Of Pharmacognosy, 06100 Tandoğan Ankara, Turkey
Shikimic acid is a phenolic acid which is known to possess several activities and it takes attention as a leading compound to some synthetic medicinal substances as well. In this study, high performance liquid chromatographic analyses were carried out in order to determine shikimic acid contents of Alnus glutinosa subsp. glutinosa, A. orientalis var. orientalis and A. orientalis var. pubescens; acetonitrile and 0.2% o-phosphoric acid: water mixture with isocratic flow was used as mobile phase. The flow rate was 0.5 ml/min. The quantitative analysis of methanol extracts prepared from the leaves of A. glutinosa subsp. glutinosa, A. orientalis var. orientalis and A. orientalis var. pubescens revealed that shikimic acid amount was found to be 0.6491%, 0.4309% and 0.2452% respectively.
Keywords: Alnus, Betulaceae, HPLC, Quantitative analysis, Shikimic acidTürkiye’de Yetişen Üç Alnus Taksonunun Metanollü Ekstrelerinde Şikimik Asit Miktar Tayini
Çiğdem Altınyay1, Burçin Ergene Öz2, Mehmet Levent Altun21The Ministry Of Health Of Turkey, Turkish Medicines And Medical Devices Agency, Ankara, Turkey2Ankara University, Faculty Of Pharmacy, Department Of Pharmacognosy, 06100 Tandoğan Ankara, Turkey
Şikimik asit, çeşitli biyolojik aktivitelere sahip ve aynı zamanda, bazı sentetik maddeler için başlangıç materyali olarak dikkat çeken bir fenolik asittir. Bu çalışmada, Alnus glutinosa subsp. glutinosa, A. orientalis var. orientalis ve A. orientalis var. pubescens ile yüksek performanslı sıvı kromatografisi analizleri yapılmış; asetonitril ve %0.2 o-fosforik asit: su karışımı izokratik akış ile mobil faz olarak kullanılmıştır. Akış hızı 0.5 ml/dk olarak verilmiştir. A. glutinosa subsp. glutinosa, A. orientalis var. orientalis ve A. orientalis var. pubescens yapraklarından hazırlanan metanol ekstrelerinde şikimik asit miktarları sırasıyla, %0.6491, %0.4309 ve %0.2452 olarak tespit edilmiştir.
Anahtar Kelimeler: Alnus, Betulaceae, YPSK, Kantitatif analiz, Şikimik asitCorresponding Author: Burçin Ergene Öz, Türkiye
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