EVALUATION OF ANTICANCER AND ANTIOXIDANT ACTIVITIES OF COFFEE STEM PARASITE EXTRACT (Scurrula ferruginea (Roxb. Ex Jack) Danser) AND IN SILICO STUDIES OF ITS ISOLATEDestria Roza1, Gianna Fadhilah2, Eka Indriani1, Yuli Asih Wiliranti1, Tita Juwitaningsih11Chemistry Study Program, Faculty Of Mathematics And Natural Sciences, Medan State University 2Department Of Chemical Engineering, National Taiwan University Of Science And Technology, Taipei, Taiwan
INTRODUCTION: Coffee parasite (Scurulla ferruginea (roxb.ex jack) Danser) has been shown to exhibit various biological activities. Based on previous pharmacological studies, coffee parasites are efficacious for treating cancer or cytotoxicity and are vasorelaxants. In this study we aims to examine the potential of the parasite Scurrula ferruginea (Roxb. Ex Jack) Danser on coffee stems as a natural anticancer. METHODS: In-silico and in-vitro studies have been carried out on coffee stem parasite extracts to analyze compounds that have the potential to act as HER2 inhibitors, the antioxidant activity of the extract, and the extract's ability to act as an anticancer agent against HeLa and MCF-7 cells. RESULTS: The research results show that several components in coffee stem parasite extract, including flavonoids and fatty acids, have the potential to act as HER2 inhibitors. The coffee stem parasite extract has strong antioxidant activity with IC50 of 59.736 ppm but is inactive against cancer cells. Characterization using gas chromatography-mass spectrometry showed the presence of bis (2-Ethylhexyl) phthalate (C24H38O4) in coffee stem parasite extract, which is toxic as an anticancer drug.
DISCUSSION AND CONCLUSION: Coffee stem parasite extract does not function as an anti-cancer agent, but its strong antioxidant activity has potential for other applications.
Keywords: anticancer, antioxidant, bis (2-Ethylhexyl) phthalate, coffee parasite, HER2 inhibitors
Corresponding Author: Destria Roza, Indonesia
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