Antioxidant Activity And Phytochemical Screening Of Some Asteraceae Plants
Filiz Bakar1, Özlem Bahadır Acıkara2, Burçin Ergene2, Serpil Nebioğlu1, Gülçin Saltan Çitoğlu21Ankara University, Faculty Of Pharmacy, Department Of Biochemistry, 06100, Tandoğan, Ankara, Turkey2Ankara University, Faculty Of Pharmacy, Department Of Pharmacognosy, 06100, Tandoğan, Ankara, Turkey
Crepis foetida subsp. rhoeadifolia, Erigeron caucasicus subsp. venustus, Hieracium bornmuelleri, Leontodon crispus var. asper, Pilosella hoppeana subsp. testimonialis and Reichardia glauca belonging to the Asteraceae family, were evaluated for their antioxidant activities and phytochemical analysis of the tested extracts was carried out in current study. Antioxidant activities were investigated by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay and measuring malondialdehyde (MDA) levels. Chemical composition of the extracts was investigated by using some standards such as; phenolic acids; chlorogenic acid, caffeic acid, ferulic acid, rosmarinic acid, p-coumaric acid and flavonoids; apigenin, luteolin, quercetin, hyperoside, rutin, hesperidin by validated HPLC method. All the tested extracts exhibited antioxidant activity in DPPH radical scavenging test. The aerial part extracts of P. hoppeana subsp. testimonialis, Crepis foetida subsp. rhoeadifolia and both aerial parts and roots of Leontodon crispus var. asper were determined as the most active. The highest inhibitory effect of Leontodon crispus var. asper roots was also revealed by measuring MDA levels. According to the phytochemical analysis chlorogenic acid was detected in all species investigated except for Reichardia glauca aerial parts.
Keywords: Asteraceae, Chlorogenic acid, DPPH, HPLC, MDAAntioxidant Activity And Phytochemical Screening Of Some Asteraceae Plants
Filiz Bakar1, Özlem Bahadır Acıkara2, Burçin Ergene2, Serpil Nebioğlu1, Gülçin Saltan Çitoğlu21Ankara University, Faculty Of Pharmacy, Department Of Biochemistry, 06100, Tandoğan, Ankara, Turkey2Ankara University, Faculty Of Pharmacy, Department Of Pharmacognosy, 06100, Tandoğan, Ankara, Turkey
Bu çalışma Asteraceae familyasına ait Crepis foetida subsp. rhoeadifolia, Erigeron caucasicus subsp. venustus, Hieracium bornmuelleri, Leontodon crispus var. asper, Pilosella hoppeana subsp. testimonialis ve Reichardia glauca türlerinin antioksidan kapasitelerini değerlendirmeyi ve etkiden sorumlu bileşiklerin belirlenebilmesi için ekstrelerin fitokimyasal içeriklerinin karakterize edilmesini amaçlamaktadır. Antioksidan aktivite 1,1-difenil-2-pikrilhidrazil (DPPH) radikal süpürücü etki ve malondialdehit (MDA) seviyelerinin ölçülmesiyle tespit edilmiştir. Test edilen ekstrelerin fitokimyasal analizleri valide edilmiş YBSK yöntemi ile bazı fenolik asit standartları (klorojenik asit, kafeik asit, ferulik asit, rozmarinik asit, p-kumarik asit) ve flavonoit standartları (apigenin, luteolin, kersetol, hiperozit, rutin, hesperidin) kullanılarak yapılmıştır. Test edilen tüm ekstreler DPPH radikal süpürücü aktivite göstermiştir. P. hoppeana subsp. testimonialis, Crepis foetida subsp. rhoeadifolia toprak üstü ve Leontodon crispus var. asper kök ve toprak üstü kısımları en aktif ekstreler olarak belirlenmiştir. Leontodon crispus var. asper kök ekstresi ile MDA seviyesinde en yüksek düşüş sağlanmıştır. Fitokimyasal analiz sonuçları Reichardia glauca toprak üstü hariç test edilen tüm ekstrelerin klorojenik asit içerdiğini ortaya koymuştur.
Anahtar Kelimeler: Asteraceae, Klorojenik asit, DPPH, YBSK, MDACorresponding Author: Özlem Bahadır Acıkara, Türkiye
| TOOLS |
 Full Text PDF Print Download citation RIS EndNote BibTex Medlars Procite Reference Manager Share with email Share Send email to author
Similar articles Google Scholar |