. 2023; 20(5): 318-327 | |||
Development and full validation of a novel liquid chromatography electrochemical detection method for simultaneous determination of nine catecholamines in rat brainSaniye Özcan1, Aysun Geven1, Murat Kozanlı2, Nafiz Öncü Can11Department of Analytical Chemistry, Faculty of Pharmacy, Anadolu University, 26470, Eskisehir, Turkey2Pharmacy Services, Health Services Vocational School, Bilecik Şeyh Edebali University, 11230, Bilecik, Turkey INTRODUCTION: Chemical neurotransmission, managed by neurotransmitters, has a crucial role in brain processes such as fear, memory, learning, and pain, or neuropathologies such as schizophrenia, epilepsy, anxiety/depression, and Parkinson’s disease. The measurement of these compounds is to elucidate the disease mechanisms and evaluate the outcomes of therapeutic interventions. However, this can be quite difficult due to various matrix effects and the problems of chromatographic separation of analytes. In the current study; for the first time, an optimized and fully validated fully according to FDA and EMA Bioanalytical Validation Guidance HPLC-EC method was developed for the simultaneous analysis of nine neurotransmitter compounds which are dopamine (DA), homovanilic acid (HVA), vanilmandelic acid (VA) serotonin (SER), 5-hydroxyindole-3-acetic acid (5-HIAA), 4-hydroxy-3-methoxyphenylglycol (MHPG), norepinephrine (NE), 3,4 dihydroxyphenylacetic acid (DOPAC) and 3-methoxytyramine (3-MT) and simultaneously determined in rat brain samples. Saniye Özcan, Aysun Geven, Murat Kozanlı, Nafiz Öncü Can. Development and full validation of a novel liquid chromatography electrochemical detection method for simultaneous determination of nine catecholamines in rat brain. . 2023; 20(5): 318-327 Corresponding Author: Nafiz Öncü Can, Türkiye |
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