Association of Mir-499, Mir-27a, and Mir-146a Polymorphisms with Susceptibility to Recurrent Spontaneous Abortion; In silico Analysis
Gholamreza Bahari1, mohsen taheri2, Mojgan Mokhtari3, Mahdiyeh Moudi4, Mahdi Majidpour5, Hossein Shahraki Ghadimi61Children and Adolescent Health Research Center, Resistant Tuberculosis Institute, Zahedan University of Medical Sciences, Zahedan, Iran.2Genetics of Non-Communicable Disease Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
3Department of Obstetrics and Gynecology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran.
4Department of Medical Biotechnology, School of Medicine, Neyshabour University of Medical Sciences, Baghroud Sq.Neyshabour, Razavi Khorasan Province, Iran.
5Clinical Immunology Research Center of Zahedan University of Medical Sciences, Zahedan, Iran
6Department of Clinical Biochemistry, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
Objective: Recurrent spontaneous abortion (RSA) is defined as two or more times pregnancy loss before 24 gestational weeks, accounting for 1-3% of fertile couples. A vast majority of Single Nucleotide Polymorphisms (SNPs) in some miRNA genes can change the miRNA: mRNA interaction and are associated with the occurrence risk of RSA. This study was designed to elucidate better the association between mir-27a, mir-499, and mir-146a polymorphisms and RSA risk.
Materials and methods: SNP genotyping of mir-27a (rs895819), mir-499(rs3746444), and mir-146a rs2910164 was performed using PCR-RFLP and Tetra-ARMS PCR methods in 98 RSA patients and 105 healthy subjects.
Results: Our results showed that the mir-499 rs3746444 and mir-27a rs895819 polymorphisms were significantly associated with RSA risk, while no significant differences were observed between rs2910164 polymorphism and RSA susceptibility.
Conclusion: we proposed that mir-499 rs3746444 and mir-27a rs895819 polymorphisms were correlated with RSA in our population, but the mir-146a rs2910164 variant is not related to the occurrence risk of RSA.
Corresponding Author: Mahdiyeh Moudi, Iran
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