. 2007; 4(3): 184-189 | |||
Immunohistochemical evaluation of VEGF, EGF-r and TGF-α In placental bed of preeclamptic pregnanciesTeksin Çırpan1, Fuat Akercan1, Mustafa Coşan Terek1, Hasan Tayfun Özçakır2, Gulsen Giray3, Sermet Sağol11Department Of Obstetrics And Gynecology, Ege University Faculty Of Medicine, Izmir, Turkey2Department Of Obstetrics And Gynecology, Celal Bayar University Faculty Of Medicine, Manisa, Turkey 3Department Of Histology And Embryology, Celal Bayar University Faculty Of Medicine, Manisa, Turkey OBJECTIVE: The aim of the study is to determine vascular endothelial growth factor (VEGF), epidermal growth factor receptor (EGF-r) and transforming growth factor alpha (TGF-α) with the immunohistochemical staining at the placental bed biopsies of patients with preeclampsia. Design: Prospective cohort study. METHODS: Biopsies from the placental bed were obtained from twelve patients with preeclapmsia and ten patients of control group during the cesarean section. The sections from the biopsies were subjected to immunohistochemical staining by using anti-VEGF, anti-EGF-r and anti-TGF-α antibodies and were examined by light microscopy. The staining intensities were graded semi-quantitatively and the H-scores were calculated by two histopatologist who were blinded to the groups. RESULTS: The VEGF expression was significantly lower in both myometrium and decidua of preeclamptic women compared to controls (77.2±25.4 versus 134±44.3, p=0.007; 170.2±17 versus 194.1±20.7, p=0.017, respectively).EGF-r expression also was significantly lower at both decidua and myometrium in the preeclamptic group (p<0.05). TGF-α expression of myometrium and decidua was not significantly different between two groups (p>0.05). CONCLUSION: VEGF and EGF-r expression is significantly lower at the placental bed in patients with preeclampsia. Keywords: EGFR, placental bed, preeclampsia, TGF-α, VEGFİmmünohistokimyasal metodla preeklamppsili hastaların plasenta yataklarında VEGF, EGF-r VE TGF-α araştırılmasıTeksin Çırpan1, Fuat Akercan1, Mustafa Coşan Terek1, Hasan Tayfun Özçakır2, Gulsen Giray3, Sermet Sağol11Ege Üniversitesi Tıp Fakültesi Kadın Hastalıkları Ve Doğum Anabilim Dalı İzmir2Celal Bayar Üniversitesi Tıp Fakültesi Kadın Hastalıkları Ve Doğum Anabilim Dalı Manisa 3Celal Bayar Üniversitesi Tıp Fakültesi Histoloji Ve Embriyoloji Anabilim Dalı Manisa AMAÇ: Preeklamptik hastaların plasental yataklarında, immünohistokimyasal boyama yöntemi ile, vasküler endotelial büyüme faktörü (VEGF), epidermal büyüme faktörü receptörü (EGF-r) ve transforming büyüme faktörü alfa’nın (TGF-α) araştırılması. Teksin Çırpan, Fuat Akercan, Mustafa Coşan Terek, Hasan Tayfun Özçakır, Gulsen Giray, Sermet Sağol. Immunohistochemical evaluation of VEGF, EGF-r and TGF-α In placental bed of preeclamptic pregnancies. . 2007; 4(3): 184-189 Corresponding Author: Teksin Çırpan, Türkiye |
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