The Influence of Certain Nutritional and Environmental Factors on The Production of Amylase Enzyme by Streptomyces Aureofaciens 77

The effect of certain nutritional and environmental factors on amylases production by the highly amylolytic Egyptian isolate Stroptomyces aureofaciens 77 was studied. The trial experiments to obtain the highest yield of amylase production resulted in modification of the recommended basal inorganic salts-starch medium. The highest yield of amylase production was obtained by increasing the soluble starch from 10g/L as a sole source of carbon up to 30 g/L and by adding corn steep liquor 20g/L as a sole source of nitrogen instead of the inorganic nitrogen source (NH4)2SO4, and growing of the used organism in the modified medium adjusted pH 7.0 under shaken culture condition for 7 days incubation at 28°C. Optimum inoculum size was found to be 4% spore supension from 6 days old culture of the S. aureofaciens 77.