Comparative Studies of Glucose Metabolism and Glycogenesis in Vitro between C3h/He and C58 Mice

The amount of glucose phosphorylated and recycled by hepatocytes prepared form
C3H/He and C58 mice was measured as an indication of the difference between the
hepatic glucokinase activity in one strain compared to the other. The glucokinase activity in one strain compared to the other. The glucokinase activity was determined spectrophotometrically and by an assessment of the in vitro consumption of (2-3H) glucose by hepatocytes. The variance in glucokinase activity between the two strains does not apparently affect the minimal rate of glucose recycling as measured by the relative loss of 3H and 14C from (2-3H, U-14C) -glucose. The correlation between glycogen synthesis and glucokinase activity in isolated hepatocyte cells is being considered.