Treatment of Involutional Osteoporosis Using Calcitonin and Bisphosphanates
Ramazan Demir1, İsmail Üstünel1, Hüseyin Bağcı2, Ayşe Yasemin Demir3, M. Halil Ertuğ41From Department of Histology and Embriyology, Faculty of Medicine, Akdeniz University, Antalya, Türkiye.2From Department of Genetics, Faculty of Medicine, Akdeniz University, Antalya, Türkiye.
3From Department of Pediatrics, Faculty of Medicine, Akdeniz University, Antalya, Türkiye.
4From Laboratory of Biochemistry, Institute of Anatomy, Medical Faculty RWTH, Aachen, Germany.
This study concerns the evaluation of -thalassemia alleles in 20 individuals with homozygous -thalassemia major, using gene amplification and dot-blot hybridization with synthetic oligonucleotide probes.
In addition to the genetic and molecular screening, erythropoietic cells in the peripheral blood samples of the same patients (12 non-splenectomized and 8 splenectomized) were studied by transmission electron microscopy. The molecular lesions causing -thalassemia major have been delineated in Antalya (a Turkish city on the Mediterranean coast) by using polymerase chain reaction technique, which preferentially amplifies -globin DNA sequences containing the most frequent -thalassemia mutations in this region.
The Mediterranean population in Antalya carries six different -globin mutations; four of these had been cumulated into IVS-I domain of -globin gene, which account for nearly 95% of all abnormalities.
On the other hand, most of the erythroblastic cells showed many intracytoplasmic alterations corresponding to that of mutations of -thalassemia.
Sorumlu Yazar: Ramazan Demir, Türkiye
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