. 1994; 7(1): 34-38 | |||
Tocopherol Contents of Pakistani Seed Oils Studied by Normal Phase HPLCFazlı MananFrom Department of Agricultural Chemistry and Nutrition, NWFP Agricultural University Peshawar, Pakistan.A high performance liquid chromatographic (HPLC) method was developed for the quantitative determination of tocopherol vitamers (, T and ) in different seed oils. After saponification the oil samples and extracts in n-hexane, tocopherol vitamers were quantitatively analyzed by HPLC with fluorimetric detector. Assay of tocopherol vitamers added to margarine resulted in •97% recovery, showing high precision of the method. The tocopherol vitamers were eluted isocratically by an eluent containing 95% hexane, 5% diethyl ether, at a pump flow rate of 1.5 ml/min. A silica column (Si-60) with a temperature of 45°C was used. The method developed in this investigation enables better separation of the tocopherol vitamers and their quantitative analysis in seed oils. Keywords: Tocopherol, Pakistani seed oils, normal phase HPLC, liquid chromatography (HP), vitamin E.Fazlı Manan. Tocopherol Contents of Pakistani Seed Oils Studied by Normal Phase HPLC. . 1994; 7(1): 34-38 Sorumlu Yazar: Fazlı Manan, Pakistan |
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