Ulus Travma Acil Cerrahi Derg. 2007; 13(3): 173-179 | |||
Effects of low dose trapidil on electrical properties of a rat peripheral nerve after crush injuryBelgin Büyükakıllı1, Bahar Taşdelen2, Sevgi Güneş11Department Of Biophysics, Medicine Faculty Of Mersin University, Mersin, Turkey.2Department Of Biostatistics, Medicine Faculty Of Mersin University, Mersin, Turkey. BACKGROUND Trapidil has been shown to possess the protective effects in the treatment of ischemia and reperfusion injury in the peripheral nervous system. The purpose of this study was to determine the effects of low dose trapidil on peripheral nerve regeneration with electrophysiological method. METHODS The sciatic nerve was compressed for 20 sec by using a jeweler’s forceps. Trapidil treatment groups were administrated a single dose of trapidil (8 mg/kg) intraperitoneally just after the injury. Electrophysiological recordings were performed in crush and crush + trapidil treatment groups on the 2nd, 7th, 15th, 30th and 45th days following the nerve injury. RESULTS EMG recordings on the second day following the crush injury showed low values of compound motor action potentials in the gastrocnemius muscle when compared to normal values obtained in intact animals; also, the values on the second day following the crush injury were significantly different between control and trapidil-treated groups. The action potential values for both groups did not yet reach baseline values at the end of the experiment. There was no difference in the action potential amplitude, area and distal latency values between rats with crush and crush+trapidil on all days. CONCLUSION We could not prove a neuroprotective effect of a single low dose of trapidil in rat crush injury model using electrophysiological method. Keywords: Compound motor action potential, crush injury; neuroprotection; peripheral nerve; trapidil.Sıçan periferik sinirinin elektriksel özellikleri üzerine hasar sonrası düşük doz trapidil tedavisinin etkileriBelgin Büyükakıllı1, Bahar Taşdelen2, Sevgi Güneş11Mersin Üniversitesi Tıp Fakültesi Biyofizik Anabilim Dalı, Mersin2Mersin Üniversitesi Tıp Fakültesi Biyoistatistik Ana Bilim Dalı, Mersin AMAÇ Bazı çalışmalarda, trapidilin periferik sinir sisteminde iskemi ve reperfüzyon hasarındaki koruyucu etkileri gösterilmiştir. Ancak, periferik sinir hasarı sonrası trapidilin etkileri değerlendirilmemiştir. Çalışmamızın amacı, düşük doz trapidil’in sinir rejenerasyonu üzerindeki etkilerini elektrofizyolojik yöntemle saptamaktır. Belgin Büyükakıllı, Bahar Taşdelen, Sevgi Güneş. Effects of low dose trapidil on electrical properties of a rat peripheral nerve after crush injury. Ulus Travma Acil Cerrahi Derg. 2007; 13(3): 173-179 Corresponding Author: Belgin Büyükakıllı, Türkiye |
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